Composite

Part:BBa_K2543009:Design

Designed by: CHUNG-HSUAN HSIAO   Group: iGEM18_Mingdao   (2018-10-08)


dToll transmembrane and intracellular domain-polyA / pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 1323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 793


Design Notes

A NgoMIV site was added in the 5' end of dToll for fusion protein construction


Source

The part was assembled with the existing parts


References